Vaccine Contamination

The following article is by Sandra Brigola of Vonhapsburg Danes and is reprinted with permission – thank you Sandra for allowing us the privilege of sharing this VERY important article. And for the record this information is just as valid today as in 2002 when this was written. In fact we’d venture to say even more so because even more “junk” is in vaccines today:

By: Sandra Brigola

It’s bad enough that we have mercury, formaldehyde, and aluminum salts added to that cocktail of poisons known as a vaccine, but do you know what else is slipping into the mix?

Did you know that there is ample scientific evidence that serious viruses, bacteria or components and toxins therefrom; as well as foreign animal or cancer related proteins and DNA are finding their way into commercial vaccines intended for humans, pets and animals?

Viruses cannot survive or reproduce without being introduced into cells that nourish them, which enables the viral reproductive activity. In that sense all viruses can be considered parasitic on other cells. Living cell types commonly used to reproduce viruses in the lab include monkey kidney cells, chicken embryos as well as other animal and human cells. These cells must also be nourished with food, and are most often fed with a nutrient containing in large part bovine (cow) serum (usually extracted from fetal calf blood). This product can carry many types of bovine blood born viruses and is one of the primary sources of vaccine contaminants.

Most commercially available bovine sera are contaminated with BOVINE DIARRHEA VIRUS and in one case I remember well was a blue tongue virus that contaminated canine vaccines. (1) One study found that 75% of all laboratory cell lines examined were contaminated with pestivirus strains, of these ALL were contaminated with one of the 3 possible BVDV strains, cell lines from other animal sources including primates contained one of these BVDV strains.

There is now heightened concern that this virus and others can cross species lines, creating new strains as they adapt to their new hosts; this would include passage of the virus to and from humans.

Scientists from the USDA National Veterinary Services Laboratory describe the situation quite clearly, and give an indication of the seriousness of the problem: ‘The high frequency of virus and antibody detection in individual animal or small pool samples suggests that any large pool of unscreened sera will be contaminated. Vaccine produced on contaminated cells may in turn be contaminated, leading to seroconversion or disease in the vaccine. (2)
Most vaccines are cultured in what is called continuous cell line cultures. These are cell cultures that continuously divide and with viral contamination in cell lines, they potentially promote cancer. How does this happen?

The virus, (which in this case has a single strand of RNA for its genome) is capable of incorporating RNA from the cells in which it is cultured, into its own genome. If any contamininant RNA virus is present in a culture that contains ‘immortal’ (grown on cell line cultures) cancerous cells, this virus can easily mutate to include unwanted cancerous material , which can then get passed into the biological product intended for human/animal use. It is well known in the scientific community that after these cells have been repeatedly cultured a certain number of times, ‘something’ causes them to convert to a cancerous state. What happens when an immortal cell from a vaccine culture makes its way into the final vaccine product; does it keep dividing in the human/animal body?

Current proposed standards for vaccines would permit contamination with up to 100pg.(picograms) of heterologous DNA PER DOSE! This is equivalent to about 10(8) ‘functional lengths’ of DNA. Note that 10(8) means qo to the power of 8, or 100,000,000 ‘functional lengths of DNA are allowed per dose of vaccine; and this is in humans; lord knows what it is for animals.

It took forty years for scientists to acknowledge that we have a serious problem as a result of the contamination of the polio vaccine with simian virus 40(SV40) from the African Green monkey, but the medical community has been slow to acknowledge a definite link between SV40 and cancer in humans. SV40 has now been found in children. Considering that children of this era are not supposed to be receiving the virus via the vaccine contamination route, this would therefore imply that SV40 is being transmitted from one human to another, in ways not previously known.

Simply put, are we in a state of denial that vaccines are ultimately transmitting viruses, DNA and proteins into humans/animals from foreign animal sources? May this be contributing to the incredible upsurge in cancers and serious chronic diseases? Are these foreign animals altering your and your animals DNA?

Other calf serum virus contamination include a virus from the parvovirus family, Bovine polyomavirus,(cancer causing) mycoplasma (68%), bovine herpes virus, parainfluenza 3- virus, Bovine leukemia virus, bovine visna virus and bovine immunodeficiency virus ultimately end up in human and animal vaccines via the use of calf serum in the manufacturing process. Note: rubella, chickenpox, smallpox, and animal multi valiant vaccines are cultured on calf serum.

Avian Leucosis Virus

Chicken eggs present another problem. (Aqua avian leukemia virus) is a retroviral pathogen that infects large segments of the modern poultry industry. It can easily transform into a dizzying array of related viruses by hijacking one of numerous cancer-related gene segments from its host, and inserting it into its own genome. Furthermore it has the additional capability of inserting itself into the host (including human/animal) genome, hiding out so to speak, and causing cancerous cell transformation from that location.

In the 1960’s it was determined that yellow fever vaccine contained it. In 2001, twelve vaccine manufacturers were reported to produce vaccines containing avian leucosis virus, including the canine distemper vaccine. (3)

Common vaccines that are grown on egg cultures are influenza, mumps, measles, yellow fever and canine distemper.

Toxin contamination

The unintentional presence of bacterial source toxins called ‘endotoxins or exotoxins‘ in human and veterinary vaccines has been recognized for many years. Such toxins are originally present in source materials or are produced as a result of bacterial infection during the manufacturing process. Diphtheria and Tetanus are specifically created to induce a protective mechanism in the body against bacterial toxin, however vaccines prepared from bacteria can contain appreciable and potentially dangerous lingering amounts of toxin, despite the steps used during manufacturing. Bacterial toxin contamination residing in calf serum can cause breaks in the DNA of human/animal cells. (4)


Nanobacteria are the smallest existing bacterial form known to science., easily escaping from the filtering process. They also have the ability to change physical form.

Any vaccine products that incorporate mammalian products during production (which would include cow, monkey or human cells, blood or serum) will be prone to nanobacterial contamination. It has been found that 2 out of 3 lots of inactivated polio vaccine and 3 out of 6 lots of veterinary vaccines were contaminated with nanobacteria.

A human variety of this pathogen has been found to cause or be associated with a host of disease conditions, only a few of which include arteriosclerosis, coronary artery/heart disease, kidney stones and kidney disease, arthritis, MS, Alzheimer’s, some cancers and other conditions. (5)

These are real sneaky bacteria forms which have the capability of transferring through the air or routine handling in labs. They have a very thin wall, described in many texts as having no cell walls and being resistant to many types of antibiotics.

Mycoplasmas have various forms and have been associated with Gulf War illness, chronic fatigue syndrome, fibromyalgia, cancer and arthritis. Mycoplasmas without question have the capability of altering cell membranes and their antigens, disrupting DNA and altering cellular metabolism both in vitro and in vivo (6)

The human and animal body has normal barriers that help to protect against infiltration by foreign agents, among them the skin, the respiratory and intestinal mucous linings and the blood brain barrier. The puncture of skin by a needle breaches that barrier. (7)

Of even more concern is the administration of vaccines nasally (through the nose) or accidental passage via that route. (8) Fields Virology Text (2001) says, “the olfactory tract has long been recognized as an alternate pathway to the CHS (central nervous system)..Olfactory neurons are unprotected by the blood brain barrier.” (Intranasal inoculation of flaviviruses may result in lethal encephalitis.) (9)

SubUnit and Naked DNA Vaccines

One of the major concerns is the unpredictability and interaction of the final vaccine product with the proteins of the DNA of the host. A document from the FDA states: ” Genetic toxicity: Integration of the plasmid DNA vaccine into the genome of the vaccinated subjects is an important theoretical risk to consider in preclinical studies. The concern is that an integrated vaccine may result in insertional mutagenesis through the activation of oncogenes or inactivation of tumor suppressor genes.

To get an idea of the scope of the operation that we are dealing with here. Consider this: Large scale cell culture operations for biotechnology products uses millions of liters of complex media and gases as well as huge quantities of organic and inorganic raw materials. These raw materials must always be assumed to contain contamination by adventitious agents. ((10). Because there is a potentially large number of human and animal viruses (or viral segments) that could be entering into the final vaccine products, it would take a equally large bank of molecular probes, as well as frequent, wide spread testing, to screen for the presence of those contaminating agents.

Let’s not forget that 100,000,000 allowable pieces of cell source DNA are allowed PER DOSE of vaccine and this DOES NOT INCLUDE THE VIRAL CONTAMINANTS.

Is this something that you want injected in your body or that of your four footed companion? I think not!!!


1.Yanagi M, Bukh J, Emerson SU, Purcell RH Contamination of commercially available fetal bovine sera with bovine viral diarrhea virus genomes; J Infect Dis 1996 Dec; 174 (6): 1324-7 PMID 8940226
2.Levings RL,Wessman SJ. Bovine viral diarrhea virus contamination of nutrient serum, cell cultures and viral vaccines. Dev Biol Stand 1991;175:177-81. PMID 1665461
3.Payne LN,Biggs PM,Chubb RC, Bowden RS. Contamination of egg-adapted canine distemper vaccine by avian leucosis virus. Vet Rec 1966 Jan 8:78 (2):45-8 PMID4285488
4.Whitaker AM, Smith EM. Effect of bacterial toxins in serum on the chromosomes of WI-38 (cell culture line) Dev, Biol Stand 1976 Dec. 13-15; 37:185-90 PMID 11178403
5.What are Nanobacteria?
6.Cell culture contamination example. Ectoplasm
7.Mattman LH.2001 Cell wall deficient forms; stealth pathogens (3rd ed) CRC Press
8.Monath TP, Cropp CB, Harison AK. Mode of entry of a neurotropic arbovirus into the central nervous system. Reinvestigation of an old controversy. Lab Invest 1983 Apr, 48 (4):399-410 PMID 630055
9.Burke DS, Monath TP, “Flaviviruses”, in Knipe DM et la (ed), 2001 Fields Virology (4th ed), Vol.1 chapter 33, page 1057 Lippincott
10.Garnick RI. Raw materials as a source of contamination in large scale cell culture. Dev Biol Stand 1998; 93:21-9. PMID 9737373
Reprinted with permission Copyright 2002 by Sandra Brigola – now deceased. Shared in loving memory of Sandra and her lifelong work on behalf of the Great Dane breed.

Excerpts from material by Benjamin McRearden
Naturally Reared Great Danes